The Puno Global Health Research Site is located in the southwest corner of Peru at the banks of Lake Titicaca at 3,825 meters above sea level. Our site offers a unique environment for the conduct of environmental health research including studies on chronic exposure to biomass fuel particulates, high altitude research including the effects of hypobaric hypoxia on the cardiovascular and pulmonary systems, and studies on heavy metal groundwater contamination. Our team members have been involved in a wide variety of global research projects over the last seven years in collaboration with Universidad Peruana Cayetano Heredia (Peru), A.B. PRISMA (Peru), and Washington University (St Louis, USA). In addition, the site has hosted undergraduates, medical students, graduate students, and post-doctoral research fellows from Johns Hopkins University and other institutions. Our site is supported by the Johns Hopkins Center for Global Health, the United States National Institutes of Health, the Bill & Melinda Gates Foundation, and with philanthropic support from Mr. William Clarke III and Mr. Kenneth Banks.